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PD values did not discriminate between patients with varying CAL values. The mean PD for healthy subjects was 1.92 mm ± 0.58, significantly lower than that of patients with periodontitis 3.55 mm ± 0.55 (P < 0.001). Criterion validity, sensitivity and specificity was evaluated using a receiver operating characteristic curve (ROC). Thirty periodontally-healthy subjects and forty patients with chronic periodontitis were studied using clinical parameters, Probing Depths (PD), Clinical Attachment Loss (CAL) and RANTES/CCL5 activity. As a biomarker, it may offer an improved prognostic and diagnostic ability compared with other biomarkers currently in use. The non-specific pro-inflammatory chemokine, RANTES/CCL5, is produced by most cells types and has an established role in periodontal disease pathogenesis. To evaluate the ability of RANTES/CCL5 to differentiate gingivitis and periodontitis. 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